Piper betle L. Leaves Extract Potentially Reduce the Nitric Oxide Production on LPS-Induced RAW 264.7 Cell Lines

Chronic inflammation can lead to several diseases that represent the leading causes of mortality worldwide. Conventional treatment carry some risks. Therefore, research on herbal medicine are suspected having anti-inflammatory effects, such as Piper betle L., is important. This study aims investigate effect P. L. extract nitric oxide a pro-inflammatory mediator. The dried leaves were extracted by ethanol. RAW 264.7 cells treated with LPS and (PBE). Nitric was measured Griess method. Antioxidant activity determined using 2,2–Diphenyl-1-picrylhydrazyl (DPPH) Total flavonoids phenolic content also identified aluminium chloride Folin–Ciocalteu colorimetric assay, respectively. demonstrated PBE has excellent NO suppression IC50 56.22±16.41 μg.mL-1, without cytotoxicity. DPPH inhibitory concentration values 279.67±11.36 ppm. Interestingly, flavonoid 50.17±3.14 mg QE.g-1 128.92±1.2 GAE.g-1. These compounds thought be responsible for its well antioxidant. proved could used candidate drugs. Nevertheless, further about biological mechanism their bioactive compounds' purification still required. Keywords: Inflammation, lipopolysaccharide, Macrophage, Oxide, betle.